International Journal of Cosmetic Science

<jats:title>Abstract</jats:title><jats:sec><jats:title>Objective</jats:title><jats:p>Porphyrins are ubiquitous metabolites and are constitutive of the bacterial metabolome of healthy skin. Their consideration has until now been limited to their pro‐inflammatory activity in <jats:italic>acne vulgaris</jats:italic>. The present work suggests a new role for these molecules in the onset of skin ageing.</jats:p></jats:sec><jats:sec><jats:title>Methods</jats:title><jats:p>A mixture of coproporphyrin III and protoporphyrin IX, representative of skin microbiota metabolites, was defined and applied in different skin models. Finally, an in vivo study was conducted to determine the association between porphyrin's abundance and ageing signs.</jats:p></jats:sec><jats:sec><jats:title>Results</jats:title><jats:p>Bacterial porphyrins penetrated <jats:italic>stratum corneum</jats:italic> and reached living epidermal cells. The porphyrin mixture increased IL‐8, ROS and melanin contents. Porphyrin‐induced melanin synthesis appeared to be regulated by translocator protein TSPO/PBR. In fibroblasts, bacterial metabolites down‐regulated a set of transcripts involved in extracellular matrix architecture and associated with ageing, which was confirmed by a decrease of type I pro‐collagen. Finally, the clinical study established positive and significant correlations between porphyrin abundance and the severity of ageing signs, including invisible spots, brown spots and wrinkles length.</jats:p></jats:sec><jats:sec><jats:title>Conclusion</jats:title><jats:p>In summary, porphyrins could play unexpected roles in skin's premature ageing process, a phenomenon we propose to call Porphyr'ageing, that promotes hyperpigmentation, inflammation, oxidative stress and fibroblast cell ageing, leading to dermal matrix weakening.</jats:p></jats:sec>

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Skin‐aging pigmentation: who is the real enemy?

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Porphyrin production by Corynebacterium diphtheriae strains from clinical isolates

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Diazepam enhances melanogenesis, melanocyte dendricity and melanosome transport via the PBR/cAMP/PKA pathway

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Health Sci Rep

Porphyrins are ubiquitous metabolites and are constitutive of the bacterial metabolome of healthy skin. Their consideration has until now been limited to their pro-inflammatory activity in acne vulgaris. The present work suggests a new role for these molecules in the onset of skin ageing.
A mixture of coproporphyrin III and protoporphyrin IX, representative of skin microbiota metabolites, was defined and applied in different skin models. Finally, an in vivo study was conducted to determine the association between porphyrin's abundance and ageing signs.
Bacterial porphyrins penetrated stratum corneum and reached living epidermal cells. The porphyrin mixture increased IL-8, ROS and melanin contents. Porphyrin-induced melanin synthesis appeared to be regulated by translocator protein TSPO/PBR. In fibroblasts, bacterial metabolites down-regulated a set of transcripts involved in extracellular matrix architecture and associated with ageing, which was confirmed by a decrease of type I pro-collagen. Finally, the clinical study established positive and significant correlations between porphyrin abundance and the severity of ageing signs, including invisible spots, brown spots and wrinkles length.
In summary, porphyrins could play unexpected roles in skin's premature ageing process, a phenomenon we propose to call Porphyr'ageing, that promotes hyperpigmentation, inflammation, oxidative stress and fibroblast cell ageing, leading to dermal matrix weakening.
Les porphyrines sont des métabolites omniprésents et constituent le métabolome bactérien de la peau saine. Leur prise en compte a jusqu’à présent été limitée à leur activité pro‐inflammatoire dans l’acné vulgaire. Le présent travail suggère un nouveau rôle pour ces molécules dans l’apparition du vieillissement cutané. MÉTHODES: Un mélange de coproporphyrine III et de protoporphyrine IX, représentatif des métabolites du microbiote cutané, a été défini et appliqué dans différents modèles cutanés. Enfin, une étude in vivo a été réalisée pour déterminer l’association entre l’abondance des porphyrines et les signes de vieillissement. RÉSULTATS: Les porphyrines bactériennes ont pénétré dans la couche cornée et atteint les cellules épidermiques vivantes. Le mélange de porphyrines a augmenté les contenus en IL‐8, ROS et mélanine. La synthèse de mélanine induite par les porphyrines semble être régulée par la protéine translocatrice TSPO/PBR. Dans les fibroblastes, les métabolites bactériens ont régulé à la baisse un ensemble de transcrits impliqués dans l’architecture de la matrice extracellulaire et associés au vieillissement, ce qui a été confirmé par une diminution du pro‐collagène de type I. Enfin, l’étude clinique a établi des corrélations positives et significatives entre l’abondance des porphyrines et la gravité des signes de vieillissement, y compris les taches invisibles, les taches brunes et la longueur des rides.
En résumé, les porphyrines pourraient jouer des rôles inattendus dans le processus de vieillissement prématuré de la peau, un phénomène que nous proposons d’appeler Porphyr’ageing, qui favorise l’hyperpigmentation, l’inflammation, le stress oxydatif et le vieillissement des cellules fibroblastes, conduisant à un affaiblissement de la matrice dermique.

Bacterial porphyrins in healthy skin: Microbiota components impact melanogenesis and age-related processes leading to Porphyr'ageing.

Bacterial porphyrins in healthy skin: Microbiota components impact melanogenesis and age‐related processes leading to Porphyr'ageing

Bacterial porphyrins in healthy skin: Microbiota components impact melanogenesis and age‐related processes leading to Porphyr'ageing

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